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</div> <div class="container-fluid clearfix layout_564394 text-center text-bold" style="font-size: 20px; color: rgb(0, 0, 0); margin-top: 30px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_439253"> <div class="column"> <div class="column visual-conent-title"> <p class="content-title"> 血清細胞外囊泡含有原發性硬化性膽管炎和膽管癌的蛋白生物標志物</p> <div class="clearfix"> </div> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_610181" style="margin-top: 0px;"> <div class="column"> <div class="container clearfix layout_148235 text-center text-bold" style="font-size: 20px; color: rgb(0, 0, 0);"> <div class="column"> <div class="hr"> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_369638 text-center" style="margin-top: 15px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_13448"> <div class="column"> <div class="col-md-12 layout_455380"> <div class="col-md-2 column"> </div> <div class="col-md-8 column"> <div class="column visual-conent-author visual-inline-block"> <p class="content-author"> <span>作者：</span>admin</p> </div> <div class="column visual-conent-adddate visual-inline-block" style="padding-left: 15px; padding-right: 15px;"> <p class="content-adddate"> <span>添加時間：</span>2019-04-15 10:07:44</p> </div> <div class="column visual-conent-source visual-inline-block"> <p class="content-source"> <span>來源：</span>永諾生物</p> </div> <div class="column visual-category-statistics visual-inline-block" style="padding-left: 15px;"> 瀏覽：<script src="/index.php?case=archive&act=view_js&aid=40"></script></div> </div> <div class="col-md-2 column"> </div> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_617573" style="margin-top: 15px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_903271"> <div class="column"> <div class="column visual-category-title"> <div class="content_tools_box"> <p class="content_tools"> <a href="javascript:CallPrint('print');">打印本文</a> <a href="javascript:doZoom(14)">小</a> <a href="javascript:doZoom(18)">中</a> <a href="javascript:doZoom(20)">大</a> </p> <div class="clearfix"> </div> </div> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_43304" style="margin-top: 15px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_784828"> <div class="column"> <div class="column visual-conent"> <p><strong>題目</strong>：Serum extracellular vesicles contain protein biomarkers for primary sclerosing cholangitis and cholangiocarcinoma</p><p><strong>期刊</strong>：Hepatology</p><p><strong>影響因子</strong>：11.711</p><p><strong>簡介</strong>：膽管癌（CCA）是一類膽道系統癌癥，預后差。原發性硬化性膽管炎（PSC）等病因是膽管癌的危險因素。肝內膽管癌（ICCA）與肝細胞癌（HCC）進行非侵入性鑒別診斷有時是困難的。 目前尚未有PSC、CCA和HCC的準確非侵入性生物標志物。為尋找新生物標志物，研究人員從CCA（n = 43）、PSC（n = 30）和HCC（n = 29）患者和健康個體（對照組，n = 32）中分離出血清細胞外囊泡（EV），并檢測其蛋白質含量。通過使用納米粒子跟蹤分析（NTA），發現HCC血清EV濃度高于其他各組。圓形形態（透射電子顯微鏡）、大?。∟TA為?180nm直徑）和標記物（免疫印跡檢測CD9、CD63和CD81）表明，大多數血清EV是外泌體。</p><p>蛋白質組圖譜（通過質譜法）顯示出多個差異表達的蛋白。這些蛋白中的幾種顯示出較高的診斷價值，CCA vs對照組的ROC曲線下最大面積（AUC）為0.878，CCA II期vs對照組為0.905，PSC vs對照組為0.789，PSC非肝硬化vs對照組為0.806， CCA vs PSC為0.796，CCA Ⅱ期vs PSC為0.956，HCC vs對照組為0.904，iCCA vs HCC為0.894。通過對人CCA細胞EV的蛋白質組學分析顯示，與正常人膽管細胞釋放的EV相比，其致癌蛋白的豐度更高。在免疫缺陷小鼠的肝臟中原位植入人CCA細胞，導致含有類似人致癌蛋白的EV在血清中釋放。</p><p style="text-align: center;"><img src="/html/upload/images/201904/15563545223814.png" width="632" height="683"/></p></div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_676599" style="margin-top: 15px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_158624"> <div class="column"> <div class="hr"> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_667472"> <div class="column"> <div class="container clearfix layout_381979"> <div class="column"> <div class=" 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